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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

32.7K Views

08:46 min

January 6th, 2015

DOI :

10.3791/52357-v

January 6th, 2015


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Keywords Membrane Proteins

Rozdziały w tym wideo

0:05

Title

1:58

Analysis of Isopropyl Beta-D-1-thiogalactopyranoside (IPTG)-induced Expression by In-gel Flourescence

3:37

Scale-up of Cell Cultures and Membrane Preparation

5:30

Solubilization and Analysis of the Membrane Fraction

6:48

Results: Representative In-gel and FSEC Analyses

8:11

Conclusion

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