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Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

11.1K Views

09:50 min

February 12th, 2015

DOI :

10.3791/52325-v

February 12th, 2015


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Organotypic 3D Tissue Culture

Rozdziały w tym wideo

0:05

Title

1:28

Culture of Organotypic Bronchial Tissue

2:04

In vitro Cigarette Smoke (CS) Exposure

3:22

Measurement of Ciliary Beating & Transepithelial Electrical Resistance (TEER)

4:20

Cytochrome P450 (CYP) 1A1/1B1 Activity

5:02

RNA Extraction

6:35

Microarray Workflow & Washing and Staining

8:07

Results: Applicability of Human Organotypic Tissue Models

9:03

Conclusion

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