Electroporation of Functional Bacterial Effectors into Mammalian Cells

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10.0K Views

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08:39 min

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January 19th, 2015

DOI :

10.3791/52296-v

January 19th, 2015

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Ryan L. Sontag¹, Cosmin Mihai², Galya Orr², Alexei Savchenko³, Tatiana Skarina³, Hong Cui³, John R. Cort¹, Joshua N. Adkins¹, Roslyn N. Brown⁴

¹Biological Sciences Division, Pacific Northwest National Laboratory, ²Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, ³Structural Proteomics Group, Ontario Center for Structural Proteomics, University of Toronto, ⁴Center for Bioproducts and Bioenergy, Washington State University

Podsumowanie

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Keywords Electroporation

Bacterial Effectors

Host pathogen Interactions

Protein Interactions

Salmonella Typhimurium

Subcellular Trafficking

Protein protein Interactions

High throughput Characterization

Rozdziały w tym wideo

0:05

Title

1:50

Electroporation

2:46

Fixation and Immunofluorescence Staining

4:21

Affinity Purification

6:11

Results: Electroporation of Salmonella Effector into HeLa Cells

7:50

Conclusion

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