JoVE Journal
Neuroscience
Masz pełny dostęp do tej treści za pośrednictwem
Nanyang Technological UniversityFlow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
52.1K Views
•
13:05 min
•
December 18th, 2014
DOI :
December 18th, 2014
•Rozdziały w tym wideo
0:05
Title
1:03
Neural Cell Harvesting
3:10
Antibody-independent Intracellular Labeling with Carboxyfluorescein Succinimidyl Ester (CFSE)
4:49
Cell-surface Antigen Staining with Fluorophore-conjugated Antibodies
5:56
Fixation and Membrane Permeabilization
7:35
Intracellular Antigen Staining with Non-fluorescent Primary Antibodies
9:30
Flow Cytometry Analysis
10:37
Results: Multicolor Flow Cytometric Analysis of Surface vs. Introcellular Antigens in Neural Cells
12:36
Conclusion
Powiązane Filmy
The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry
15.4K Views
High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
16.2K Views
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
12.2K Views
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
16.8K Views
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
23.7K Views
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
7.0K Views
Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
13.5K Views
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
3.3K Views
Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives
4.0K Views
Quantification of Microglial Engulfment of Synaptic Material Using Flow Cytometry
985 Views
ISSN 1940-087X
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone
Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.
Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.