Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

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39.3K Views

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09:27 min

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March 23rd, 2015

DOI :

10.3791/52076-v

March 23rd, 2015

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Thomas Lampl¹, Jo A. Crum¹, Taylor A. Davis¹, Carol Milligan²^,³^,⁴, Victoria Del Gaizo Moore¹

¹Chemistry Department, Elon University, ²Department of Neurobiology and Anatomy, Wake Forest School of Medicine, ³Neuroscience Graduate Program, Wake Forest School of Medicine, ⁴ALS Center Translational Science Unit, Wake Forest School of Medicine

Podsumowanie

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Keywords Mitochondria Isolation

Mitochondrial Function

Cell Culture

Mouse Tissue

TMRE

Mitochondrial Uncouplers

Mitochondrial Inhibitors

Fluorescent Plate Reader

High throughput Analysis

Organelle specific Function

Cell Metabolism

Energy Production

Apoptosis

Rozdziały w tym wideo

0:05

Title

1:49

Cell Culture

2:24

Isolation of Mitochondria from Cultured Cells

4:58

Mitochondria Isolation from Mouse Tissue

6:26

Assessment of Mitochondrial (Dys)function

7:42

Results: Cytokine Treatment Leads to Increased Cell Death and Decreased Membrane Potential in Mitochondria

8:51

Conclusion

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