Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

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9.8K Views

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08:11 min

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October 14th, 2014

DOI :

10.3791/51494-v

October 14th, 2014

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Antón Barreiro-Iglesias¹, Guixin Zhang², Michael E. Selzer²^,³, Michael I. Shifman²

¹Centre for Neuroregeneration, School of Biomedical Sciences, University of Edinburgh, ²Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation), Temple University School of Medicine, ³Department of Neurology, Temple University School of Medicine

Podsumowanie

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Keywords Spinal Cord Injury

Regeneration

Wholemount In Situ Hybridization

Neuron intrinsic Mechanisms

Rozdziały w tym wideo

0:05

Title

1:22

Preparing the Larval Sea Lamprey

2:17

Complete Spinal Cord Transection

4:30

Brain Dissection

6:55

Results: Expression of neogenin After Spinal Cord Transection

7:35

Conclusion

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