Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
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10:51 min
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April 10th, 2014
DOI :
April 10th, 2014
•Francisco-Jose Fernandez-Gomez1, Fanny Jumeau1,2, Maxime Derisbourg1, Sylvie Burnouf1, Hélène Tran1, Sabiha Eddarkaoui1, Hélène Obriot1, Virginie Dutoit-Lefevre3, Vincent Deramecourt4, Valérie Mitchell2, Didier Lefranc3, Malika Hamdane1, David Blum1, Luc Buée1, Valérie Buée-Scherrer1, Nicolas Sergeant1
Rozdziały w tym wideo
0:05
Title
1:08
Extracting Total Protein
3:07
Quality Control and Dye Conjugation
5:28
Fluorescence Difference Gel Electrophoresis (2D-DIGE)
7:36
Mini-2DE for Quantitative Analysis
8:53
Proteomics of Human and Mouse Tissue with Alzheimer Disease
10:26
Conclusion
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