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A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

17.8K Views

08:26 min

April 9th, 2013

DOI :

10.3791/50410-v

April 9th, 2013


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Keywords Neutrophils

Rozdziały w tym wideo

0:05

Title

1:25

Isolation of Neutrophils (PMN) from Human Blood

3:02

Activating PMN

4:03

Isolation and Fixation of Nuclei

4:51

Nuclei Immunolabeling for Flow Cytometry Analysis

6:15

Flow Cytometry Analysis

6:49

Results: Monitoring NF-κB Dynamics

7:54

Conclusion

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