JoVE Journal

Biology

Author Produced

This content is Open Access.

Design and Use of Multiplexed Chemostat Arrays

We developed and validated a small-footprint array of miniature chemostats built from readily available parts for low cost. Physiological and experimental evolution results were similar to larger volume chemostats. The ministat array provides a compact, inexpensive, and accessible platform for traditional chemostat experiments, functional genomics, and chemical screening applications.

Rozdziały w tym wideo

0:01

Title

1:18

How the Ministats Work

2:03

Anatomy of the Array

3:11

How to Clean and Assemble a Ministat Array

3:40

Marking Culture Tubes at the Desired Working Culture Volume

4:11

Making the Cork Assembly

5:48

Setting Up the Gas Washing Bottle to Hydrate Air and Prevent Evaporation

6:39

Use of a 4-Port Manifold to Divide Air Flow Between Multiple Chambers

7:35

Setting the Peristaltic Pump and Heating Block

8:24

Air Tubing Assembly

9:08

Media Tubing Assembly

11:01

Part Assembly and Autoclaving

11:34

Connecting all Tubes to the Culture Chamber

12:33

Preparing the Carboy and Autoclaving

12:55

Running Experiments

15:13

Time Zero Sampling

16:08

Daily Measurements

16:52

Taking Down an Experiment and Cleaning Up

1:12

Design and Use of Multiplexed Chemostat Arrays

8:50

Effluent Tube Assembly

10:21

Preparing Effluent Sampling Containers

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