Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction

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10.6K Views

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11:12 min

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January 11th, 2013

DOI :

10.3791/4460-v

January 11th, 2013

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Monika S. Brill¹, Petar Marinković¹, Thomas Misgeld¹^,²^,³^,⁴

¹Lehrstuhl für Biomolekulare Sensoren, Technische Universität München, ²Center for Integrated Protein Science (Munich) at the Institute of Neuroscience, Technische Universität München, ³TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases, Technische Universität München, ⁴Munich Cluster for Systems Neurology (SyNergy), Technische Universität München

Podsumowanie

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Sequential Photo bleaching

Schwann Cells

Neuromuscular Junction

Myelinating Axonal SCs

Nodes Of Ranvier

Saltatory Action Potential Propagation

Non myelinating Terminal SCs

Light Microscopy

Dye Filling

Time lapse Recordings

Multi spectral Labeling

Rozdziały w tym wideo

0:05

Title

1:36

Preparing the Triangularis Sterni Explant

4:34

Photobleaching and Time-lapse Microscopy

7:52

Fixing and Dissecting the Explant for Immunohistochemistry

9:45

Triangularis Sterni Visualization with GFP in Schwann Cells

10:42

Conclusion

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