A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

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16.5K Views

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10:45 min

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July 20th, 2012

DOI :

10.3791/3991-v

July 20th, 2012

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Alenka Lovy¹, Anthony J.A. Molina², Fernanda M. Cerqueira³, Kyle Trudeau³, Orian S. Shirihai³

¹Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, ²Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, ³Department of Medicine, Boston University Medical Center

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:33

Imaging Plate Preparation

2:29

Imaging Parameters for the Zeiss LSM 710 Confocal Microscope

3:37

Optimizing the Imaging Parameters

4:49

Mitochondrial-fusion Assay Set-up

7:36

Analyzing the PA-GFP Signal Intensity

8:17

Results: Representative Mitochondrial-fusion Assays

9:49

Conclusion

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