Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)

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16.1K Views

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10:59 min

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April 6th, 2012

DOI :

10.3791/3513-v

April 6th, 2012

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Catherine Marquer¹, Sandrine Lévêque-Fort²^,³, Marie-Claude Potier¹

¹Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière, Hôpital de la Pitié-Salpêtrière, ²Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, ³Centre de Photonique Biomédicale du Centre Laser, Université Paris-Sud

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:48

Calibration of the FCS Setup

3:45

FCS Data Analysis

5:50

Staining of Living Cells with Lipid-rafts Marker

6:51

FCS Data Acquisition on Living Cells

8:07

Representative FCS Analysis

10:18

Conclusion

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