A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Please note that all translations are automatically generated. Click here for the English version.

16.2K Views

•

04:35 min

•

July 26th, 2011

DOI :

10.3791/2807-v

July 26th, 2011

•

Jennifer Selever¹, Jian-Qiang Kong², Benjamin R. Arenkiel³^,⁴

¹Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), ²Precisionary Instruments Inc., ³Departments of Molecular & Human Genetics and Neuroscience, Baylor College of Medicine (BCM), ⁴Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

Podsumowanie

Przeglądaj więcej filmów

High resolution Fluorescence Imaging

Semi thick Brain Slices

Neuronal Morphologies

Molecular And Cellular Makeup

Transgenic And Gene Targeting Technologies

Fluorescent Proteins

Marine Invertebrates

Cell Type specific Promoter Activity

Brain Structure And Function

Deep Brain Tissues

Rozdziały w tym wideo

0:05

Title

1:01

Agarose Embedding of Fixed Mouse Brain Tissue

1:55

Sectioning the Embedded Brain Tissue Using the Compresstome

2:33

Optical Clearing of the Brain Tissue Sections

3:14

Mounting the Brain Tissue Sections for Imaging

3:53

Epifluorescent and Confocal Microscopy of Intact Thick Brain Slices

4:16

Conclusion

Powiązane Filmy

12:15

Double Fluorescence in situ Hybridization in Fresh Brain Sections

16.0K Views

10:41

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

11.9K Views

10:06

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

12.8K Views

11:03

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

9.2K Views

08:16

High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem

15.3K Views

12:15

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

12.2K Views

10:30

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

11.6K Views

10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

10.6K Views

07:57

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

6.8K Views

09:42

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

10.8K Views