Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
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21.2K Views
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10:43 min
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December 3rd, 2010
DOI :
December 3rd, 2010
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Rozdziały w tym wideo
0:05
Title
0:51
Introduction
1:08
Protein Overexpression
2:48
Harvesting and Extracting the Cells
4:58
Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
6:46
Hexahistidine Tag Affinity Purification by IMAC
8:23
Gel Filtration
9:44
Representative Results from MexB Purification
10:23
Conclusion
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