biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

neuronal culture techniques

This collection features a variety of neuronal culture techniques, ranging from basic neuron cultures to advanced protocols for generating neural progenitors. It covers specific neuron cultures that employ three-dimensional scaffolds or microfluidics, and differentiation into brain organoids and networks. Techniques also encompass co-cultures to study neuron-glia interactions and synapse formation, among other approaches for exploring neuronal behavior and neurogenesis in vitro.

Neuronal Culture Techniques

neurophysiology

This collection presents methodologies for studying neural function and behavior. It features direct measurement techniques for recording electrical activities from neurons in diverse settings, and optical techniques for visualizing dynamic neuronal processes, tracking real-time changes, and examining spatial relationships within neural circuits. Additionally, it provides various stimulation methods using electrical, magnetic, and optogenetic tools to explore and modulate neural circuit responsiveness.

Neurophysiology

neuroimaging

This collection offers advanced methodologies for investigating brain structure, functional connectivity, and molecular dynamics in the nervous system. Focusing on in vitro and in vivo models, advanced imaging, and analytical techniques, these protocols equip researchers with innovative tools to study neural activity and pathologies across diverse areas.

Neuroimaging

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neuropathology

This collection offers advanced methodologies for investigating molecular mechanisms, structural changes, and functional disruptions in nervous system disorders. Focusing on in vitro and in vivo models, advanced imaging, and molecular analyses, these protocols equip researchers with innovative tools to study brain and neural pathologies across diverse areas.

Neuropathology

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structural & functional brain imaging

Structural & Functional Brain Imaging

synaptic & cellular imaging

Synaptic & Cellular Imaging

neurovascular & blood flow imaging

Neurovascular & Blood Flow Imaging

neurodegeneration & disease monitoring

Neurodegeneration & Disease Monitoring

developmental & regenerative neuroimaging

Developmental & Regenerative Neuroimaging

live imaging & in vivo tracking

Live Imaging & In Vivo Tracking

advanced microscopy & imaging technologies

Advanced Microscopy & Imaging Technologies

imaging hippocampal neuronal activation in mouse brain sections

imaging-hippocampal-neuronal-activation-in-mouse-brain-sections

Imaging Hippocampal Neuronal Activation In Mouse Brain Sections

Source: Provenzano, G., et al.&#160;Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of ...

investigating the origins of sensory neurons in the dura mater using fluorescence microscopy

investigating-origins-sensory-neurons-dura-mater-using-fluorescence

Investigating the Origins of Sensory Neurons in the Dura Mater Using Fluorescence Microscopy

Source:&#160;Wang, J.,&#160;et al. Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with ...

embedding drosophila brain in a matrix for time-lapse imaging of the circadian clock neurons

embedding-drosophila-brain-matrix-for-time-lapse-imaging-circadian

Embedding Drosophila Brain in a Matrix for Time-Lapse Imaging of the Circadian Clock Neurons

Source: Sabado, V.,&#160;et al.,&#160;Nagoshi, E. Single-cell Resolution Fluorescence Live Imaging of&#160;Drosophila&#160;Circadian Clocks in Larval ...

visualization of calcium influx in ventral midbrain neurons derived from mouse embryos

visualization-calcium-influx-ventral-midbrain-neurons-derived-from

Visualization of Calcium Influx in Ventral Midbrain Neurons Derived from Mouse Embryos

Source:&#160;Bancroft, E. A.,&#160;et al. Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons.&#160;J. Vis. ...

time-lapse imaging of cortical neuron radial migration in transduced mouse embryonic brain slices

time-lapse-imaging-cortical-neuron-radial-migration-transduced-mouse

Time-Lapse Imaging of Cortical Neuron Radial Migration in Transduced Mouse Embryonic Brain Slices

Source:&#160;Wiegreffe, C.,&#160;et. al.,&#160;&#160;Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse ...

dual fluorescence imaging to identify differentiated oligodendrocytes

dual-fluorescence-imaging-to-identify-differentiated-oligodendrocytes

Dual Fluorescence Imaging to Identify Differentiated Oligodendrocytes

Source:&#160;Schott, J. T.,&#160;et al. Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using ...

in vitro calcium imaging in short-term and long-term rat hippocampal cultures

in-vitro-calcium-imaging-short-term-long-term-rat-hippocampal

In Vitro Calcium Imaging in Short-Term and Long-Term Rat Hippocampal Cultures

Source:&#160;Calvo-Rodr&#237;guez,&#160;et al. Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons.&#160;J. Vis. Exp. ...

blood oxygen level-dependent functional magnetic resonance imaging of the visual cortex

blood-oxygen-level-dependent-functional-magnetic-resonance-imaging

Blood Oxygen Level-Dependent Functional Magnetic Resonance Imaging of the Visual Cortex

Source:&#160;Galenchik-Chan, A.,&#160;et al. Functional Magnetic Resonance Imaging (fMRI) of the Visual Cortex with Wide-View Retinotopic Stimulation. J. ...

functional near-infrared spectroscopy for recording brain activity during social interactions

functional-near-infrared-spectroscopy-for-recording-brain-activity

Functional Near-Infrared Spectroscopy for Recording Brain Activity During Social Interactions

Source:&#160;Reindl, V.,&#160;et al. Conducting Hyperscanning Experiments with Functional Near-Infrared Spectroscopy. J. Vis. Exp. (2019)&#160;This video ...

magnetic resonance imaging of cerebral aneurysms using mprage

magnetic-resonance-imaging-of-cerebral-aneurysms-using-mprage

Magnetic Resonance Imaging of Cerebral Aneurysms Using MPRAGE

single photon emission computed tomography for assessing brain function disruption

single-photon-emission-computed-tomography-for-assessing-brain

Single Photon Emission Computed Tomography for Assessing Brain Function Disruption

measuring cerebral blood volume in the auditory cortex using vascular space occupancy

measuring-cerebral-blood-volume-auditory-cortex-using-vascular-space

Measuring Cerebral Blood Volume in the Auditory Cortex Using Vascular Space Occupancy

transmission electron microscopic imaging of synaptic vesicle endocytosis in mouse hippocampal neurons 

transmission-electron-microscopic-imaging-synaptic-vesicle

Transmission Electron Microscopic Imaging of Synaptic Vesicle Endocytosis in Mouse Hippocampal Neurons 

Source: Villarreal, S., et. al., Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons. J. Vis. Exp. (2017)This video demonstrates the ...

immunofluorescence microscopy of neural cells differentiated from peripheral blood-derived cells 

immunofluorescence-microscopy-neural-cells-differentiated-from

Immunofluorescence Microscopy of Neural Cells Differentiated from Peripheral Blood-Derived Cells 

Source: Becker-Koji&#263;, Z. A., et. al., GM-Free Generation of Blood-Derived Neuronal Cells. J. Vis. Exp. (2021)The video demonstrates a protocol for ...

imaging intracellular atp dynamics using fret-based sensors in an organotypic mouse hippocampal slice

imaging-intracellular-atp-dynamics-using-fret-based-sensors-an

Imaging Intracellular ATP Dynamics Using FRET-Based Sensors in an Organotypic Mouse Hippocampal Slice

Source: Lerchundi, R., et. al. Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK. J. ...

microscopic analysis of synapses in mouse hippocampal slices using immunofluorescence

microscopic-analysis-synapses-mouse-hippocampal-slices-using

Microscopic Analysis of Synapses in Mouse Hippocampal Slices Using Immunofluorescence

Source: McLeod, F., et al. Evaluation of Synapse Density in Hippocampal Rodent Brain Slices. J. Vis. Exp. (2017).This video demonstrates a method for ...

two-color stimulated raman scattering imaging of mouse brain tissue

two-color-stimulated-raman-scattering-imaging-of-mouse-brain-tissue

Two-Color Stimulated Raman Scattering Imaging of Mouse Brain Tissue

Source:&#160;Espinoza, R.,&#160;et al. Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis.&#160;J. Vis. Exp. ...

histochemical imaging of cortical modules in flattened brain sections

histochemical-imaging-of-cortical-modules-in-flattened-brain-sections

Histochemical Imaging of Cortical Modules in Flattened Brain Sections

Source:&#160;Lauer, S. M.,&#160;et al.&#160;Visualization of Cortical Modules in Flattened Mammalian Cortices.&#160;J. Vis. Exp.&#160;(2018)&#160;This ...

analysis of immune cells in mouse nervous tissues using flow cytometry

analysis-of-immune-cells-in-mouse-nervous-tissues-using-flow-cytometry

Analysis of Immune Cells in Mouse Nervous Tissues Using Flow Cytometry

Source:&#160;Hidmark, A. S.,&#160;et al.&#160;Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow ...

selective single-neuron laser ablation in a zebrafish larva using a confocal microscope

selective-single-neuron-laser-ablation-zebrafish-larva-using-confocal

Selective Single-Neuron Laser Ablation in a Zebrafish Larva Using a Confocal Microscope

Source:&#160;Morsch, M.,&#160;&#160;et. al.,&#160;Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy.&#160;J. Vis. Exp. ...

 imaging polysomes isolated from a mouse brain using atomic force microscopy

imaging-polysomes-isolated-from-mouse-brain-using-atomic-force

 Imaging Polysomes Isolated from a Mouse Brain Using Atomic Force Microscopy

Source: Lunelli, L.,&#160;et. al. Peering at Brain Polysomes with Atomic Force Microscopy.&#160;J. Vis. Exp.(2016).This video demonstrates the imaging of ...

measuring protein expression in the rodent brain using near-infrared fluorescence and high-resolution scanning 

measuring-protein-expression-rodent-brain-using-near-infrared

Measuring Protein Expression in the Rodent Brain Using Near-Infrared Fluorescence and High-Resolution Scanning 

Source:&#160;Kimmelmann-Shultz, B.,&#160;et. al.&#160;Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the ...

visualization of mitochondria within intraepidermal nerve fibers

visualization-of-mitochondria-within-intraepidermal-nerve-fibers

Visualization of Mitochondria within Intraepidermal Nerve Fibers

Source:&#160;Hamid, H. S.,&#160;et al. Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers.&#160;J. Vis. ...

 imaging of neuronal mitochondria using a serial block-face scanning electron microscope

imaging-neuronal-mitochondria-using-serial-block-face-scanning

 Imaging of Neuronal Mitochondria Using a Serial Block-Face Scanning Electron Microscope

Source:&#160;Mukherjee, K.,&#160;et. al. Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy.&#160;J. Vis. Exp. (2016)This ...

two-photon microscopy for imaging neuronal morphology  in a mouse pup

two-photon-microscopy-for-imaging-neuronal-morphology-in-a-mouse-pup

Two-Photon Microscopy for Imaging Neuronal Morphology  in a Mouse Pup

Source:&#160;Mizuno, H.,&#160;et al.&#160;In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice.&#160;J. Vis. Exp. (2018)This video showcases ...

bioluminescence imaging for studying calcium transients in drosophila brain structures

bioluminescence-imaging-for-studying-calcium-transients-drosophila

Bioluminescence Imaging for Studying Calcium Transients in Drosophila Brain Structures

Source:&#160;Lark, A. R.,&#160;et. al.,&#160; In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin.&#160;J. ...

targeted neuronal ablation using two-photon microscopy in a zebrafish larava

targeted-neuronal-ablation-using-two-photon-microscopy-zebrafish

Targeted Neuronal Ablation Using Two-Photon Microscopy in a Zebrafish Larava

Source:&#160;Muto, A.,&#160;et al. Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral ...

quantifying newly generated neurons in rat brain tissue using the optical fractionator technique

quantifying-newly-generated-neurons-rat-brain-tissue-using-optical

Quantifying Newly Generated Neurons in Rat Brain Tissue Using the Optical Fractionator Technique

Source: Olesen, M. V.,&#160;et al. The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy.&#160;J. Vis. ...

identification of mouse retinal ganglion cell subtypes through fluorescent tracing

identification-mouse-retinal-ganglion-cell-subtypes-through

Identification of Mouse Retinal Ganglion Cell Subtypes Through Fluorescent Tracing

Source:&#160;Laboissonniere,&#160;et. al. Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells.&#160;J. Vis. Exp. (2017).&#160;This video ...

imaging of fluorescently-labeled motor neurons in an adult drosophila leg

imaging-fluorescently-labeled-motor-neurons-an-adult-drosophila

Imaging of Fluorescently-Labeled Motor Neurons in an Adult Drosophila Leg

Source:&#160;Guan, W.,&#160;et. al. Visualize&#160;Drosophila&#160;Leg Motor Neuron Axons Through the Adult Cuticle.&#160;J. Vis. Exp. (2018)&#160;This ...

focused ion beam scanning electron microscopy for 3d reconstruction of synaptically linked neurons

focused-ion-beam-scanning-electron-microscopy-for-3d-reconstruction

Focused Ion Beam Scanning Electron Microscopy for 3D Reconstruction of Synaptically Linked Neurons

Source:&#160;Maco B,&#160;et al. Correlative&#160;In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons.&#160;PLoS ...

 phase contrast magnetic resonance imaging to assess blood flow in the arteries of a rat

phase-contrast-magnetic-resonance-imaging-to-assess-blood-flow

 Phase Contrast Magnetic Resonance Imaging to Assess Blood Flow in the Arteries of a Rat

Source: Chiu, S., et al. Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery. J. Vis. Exp. (2018).This video demonstrates phase ...

functional imaging of cerebral blood flow using transcranial doppler ultrasound

functional-imaging-cerebral-blood-flow-using-transcranial-doppler

Functional Imaging of Cerebral Blood Flow Using Transcranial Doppler Ultrasound

Source:&#160;Hage, B. D.&#160;et. al., Functional Transcranial Doppler Ultrasound for Monitoring Cerebral Blood Flow.&#160;J. Vis. Exp. (2021)&#160;This ...

developing a cerebral blood flow atlas for blood flow mapping

developing-a-cerebral-blood-flow-atlas-for-blood-flow-mapping

Developing a Cerebral Blood Flow Atlas for Blood Flow Mapping

Source:&#160;Tan, Z.,&#160;et al., Construction and Application of Cerebral Functional Region-Based Cerebral Blood Flow Atlas Using Magnetic Resonance ...

 two-photon microscopy for in vivo imaging of mouse brain microvasculature 

two-photon-microscopy-for-vivo-imaging-mouse-brain

 Two-Photon Microscopy for In Vivo Imaging of Mouse Brain Microvasculature 

Source:&#160;Nishimura, C.&#160;et al. Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced ...

time-of-flight magnetic resonance angiography to visualize blood vasculature

time-flight-magnetic-resonance-angiography-to-visualize-blood

Time-of-Flight Magnetic Resonance Angiography to Visualize Blood Vasculature

magnetic resonance imaging of perivascular spaces in the human brain during a small vessel stroke

magnetic-resonance-imaging-perivascular-spaces-human-brain-during

Magnetic Resonance Imaging of Perivascular Spaces in the Human Brain During a Small Vessel Stroke

detection of internal carotid artery narrowing using digital subtraction angiography

detection-internal-carotid-artery-narrowing-using-digital-subtraction

Detection of Internal Carotid Artery Narrowing Using Digital Subtraction Angiography

dark blood magnetic resonance imaging for visualizing inflamed intracranial arteries

dark-blood-magnetic-resonance-imaging-for-visualizing-inflamed

Dark Blood Magnetic Resonance Imaging for Visualizing Inflamed Intracranial Arteries

bioluminescence imaging of neuroinflammation in a transgenic mouse

bioluminescence-imaging-of-neuroinflammation-in-a-transgenic-mouse

Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse

Source: Breid, S., et. al. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. ...

analyzing amyloid structures in a tissue section using fluorescence lifetime imaging microscopy

analyzing-amyloid-structures-tissue-section-using-fluorescence

Analyzing Amyloid Structures in a Tissue Section Using Fluorescence Lifetime Imaging Microscopy

Source: Nystr&#246;m, S., et al.,&#160;Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy ...

in vivo imaging of retinal microglia in a mouse model of glaucoma

in-vivo-imaging-of-retinal-microglia-in-a-mouse-model-of-glaucoma

In Vivo Imaging of Retinal Microglia in a Mouse Model of Glaucoma

Source:&#160;Bosco, A.,&#160;et al. In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell ...

Encyclopedia Of Experiments - neuroscience