less-invasive-technique-for-non-stabilized-mandibular-fracture-mouse

Less-Invasive Technique for Non-stabilized Mandibular Fracture in Mouse Models

Non-stabilized fractures can be made at mandibular sites in mice, thus making it possible to analyze bone repair using an endochondral ossification mode. ...

Université de Paris Cité

Hypochondroplasia (HCH) is a mild dwarfism caused by missense mutations in fibroblast growth factor receptor 3 (FGFR3), with the majority of cases resulting from a heterozygous p.Asn540Lys gain-of-function mutation. Here, we report the generation and characterization of the first mouse model (Fgfr3Asn534Lys/+) of HCH to our knowledge. Fgfr3Asn534Lys/+ mice exhibited progressive dwarfism and impairment of the synchondroses of the cranial base, resulting in defective formation of the foramen magnum. The appendicular and axial skeletons were both severely affected and we demonstrated an important role of FGFR3 in regulation of cortical and trabecular bone structure. Trabecular bone mineral density (BMD) of long bones and vertebral bodies was decreased, but cortical BMD increased with age in both tibiae and femurs. These results demonstrate that bones in Fgfr3Asn534Lys/+ mice, due to FGFR3 activation, exhibit some characteristics of osteoporosis. The present findings emphasize the detrimental effect of gain-of-function mutations in the Fgfr3 gene on long bone modeling during both developmental and aging processes, with potential implications for the management of elderly patients with hypochondroplasia and osteoporosis.

Anne Morice

Laboratory of Molecular and Physiopathological Bases of Osteochondrodysplasia,
Imagine Institute,
Laboratory of Molecular and Physiopathological Bases of Osteochondrodysplasia, Imagine Institute

Université de Paris Cité

Less-Invasive Technique for Non-stabilized Mandibular Fracture in Mouse Models

Anne Morice

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Laboratory of Molecular and Physiopathological Bases of Osteochondrodysplasia,Imagine Institute,Laboratory of Molecular and Physiopathological Bases of Osteochondrodysplasia, Imagine Institute

Université de Paris Cité

Less-Invasive Technique for Non-stabilized Mandibular Fracture in Mouse Models

Laboratory of Molecular and Physiopathological Bases of Osteochondrodysplasia,
Imagine Institute,
Laboratory of Molecular and Physiopathological Bases of Osteochondrodysplasia, Imagine Institute