<h2 class="jove_title"><a href="https://www-jove-com.remotexs.ntu.edu.sg/video/56990">Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation</a></h2>Ming Li1,2, Michelle Bui1,2, Omar S. Akbari1,21<a href='/institutions/NA-North-America/US-United-States/CA-California/261-University-of-California'>Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology, University of California, Riverside</a>, 2Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San DiegoMicroinjection of Nasonia vitripennis embryos is an essential method for generating heritable genome modifications. Described here is a detailed procedure for microinjection and transplantation of Nasonia vitripennis embryos, which will greatly facilitate future genome manipulation in this organism.<h2 class="jove_title"><a href="https://www-jove-com.remotexs.ntu.edu.sg/video/56737">Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb</a></h2>Joakim Henricson1,2, Rani Toll John1,3, Chris D. Anderson4,5, Daniel Bj&#246;rk Wilhelms1,21<a href='/institutions/EU-Europe/SE-Sweden/26259-Region-Ostergotland'>Department of Emergency Medicine, Local Health Care Services in Central &Ouml;sterg&ouml;tland, Region &Ouml;sterg&ouml;tland</a>, 2<a href='/institutions/EU-Europe/SE-Sweden/25348-Linkoping-University'>Division of Drug Research, Department of Medical and Health Sciences, Faculty of Health Sciences, Link&ouml;ping University</a>, 3<a href='/institutions/EU-Europe/SE-Sweden/25348-Linkoping-University'>Division of Neuro and Inflammation Science, Department of Clinical and Experimental Medicine, Link&ouml;ping University</a>, 4<a href='/institutions/EU-Europe/SE-Sweden/25348-Linkoping-University'>Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Link&ouml;ping University</a>, 5<a href='/institutions/EU-Europe/SE-Sweden/26259-Region-Ostergotland'>Department of Dermatology and Venerology, Heart and Medicine Center, Region &Ouml;sterg&ouml;tland</a>This protocol describes how the use of diffuse polarization spectroscopy can improve the clinical usefulness of the capillary refill test. We suggest a more detailed analysis of the course of the capillary refill in healthy volunteers using diffuse reflectance spectroscopy videos and new informatic endpoints.<h2 class="jove_title"><a href="https://www-jove-com.remotexs.ntu.edu.sg/video/56365">MRI-guided Focused Ultrasound Thalamotomy for Patients with Medically-refractory Essential Tremor</a></h2>Ying Meng1, Yuexi Huang2, Benjamin Solomon2, Kullervo Hynynen2, Nadia Scantlebury1, Michael L. Schwartz1, Nir Lipsman11<a href='/institutions/NA-North-America/CA-Canada/25018-Sunnybrook-Health-Sciences-Centre'>Division of Neurosurgery, Sunnybrook Health Sciences Centre</a>, 2<a href='/institutions/NA-North-America/CA-Canada/25018-Sunnybrook-Health-Sciences-Centre'>Sunnybrook Research Institute, Sunnybrook Health Sciences Centre</a>High-intensity MRI guided focused ultrasound is an emerging noninvasive technique to precisely ablate brain tissue. It has been shown to be safe and effective in treating medically-refractory essential tremor. This article describes the protocol for thalamotomy from patient selection to equipment setup to post-treatment follow-up. <h2 class="jove_title"><a href="https://www-jove-com.remotexs.ntu.edu.sg/video/54807">Laser-heating and Radiance Spectrometry for the Study of Nuclear Materials in Conditions Simulating a Nuclear Power Plant Accident</a></h2>Dario Manara1, Luca Soldi1,2,4, Sara Mastromarino1,3,5, Kostantinos Boboridis1, Davide Robba1, Luka Vlahovic1, Rudy Konings11<a href='/institutions/EU-Europe/DE-Germany/26656-European-Commission,-Joint-Research-Centre'>European Commission, Joint Research Centre</a>, 2<a href='/institutions/EU-Europe/IT-Italy/25509-Politecnico-di-Milano'>Energy Department, Politecnico di Milano</a>, 3<a href='/institutions/EU-Europe/IT-Italy/26658-Sapienza---Universita-di-Roma'>Department of Chemical Physics, Sapienza - Universit&#224; di Roma</a>, 4<a href='/institutions/EU-Europe/FR-France/26657-CEA-Saclay'>CEA Saclay</a>, 5<a href='/institutions/EU-Europe/NL-Netherlands/25443-Delft-University-of-Technology'>TU Delft</a>We present experiments in which real nuclear fuel, cladding, and containment materials are laser heated to temperatures beyond 3,000 K while their behavior is studied by radiance spectroscopy and thermal analysis. These experiments simulate, on a laboratory scale, the formation of a lava-phase following a nuclear reactor core meltdown.

jove monthly highlights december 2017

Here's a look at what's coming up in the December 2017 issue of JoVE: The World's Premier Video Journal.
From a flurry of fantastic articles for December, we begin with a customizable MRI guided focused ultrasound treatment for patients with essential tremor. In JoVE Medicine, our authors describe patient selection, equipment setup, sonication, and post treatment follow-up. The exciting results show that patients experienced both short- and long-term reductions in their tremors, beginning immediately after treatment.
Next, JoVE Neuroscience loops in to the circulatory system. Here, our authors describe a protocol that uses diffuse polarization spectroscopy to improve the clinical usefulness of the capillary refill test. The capillary refilling response is measured quantitatively using videos, in contrast to traditional methods that rely on visual observation. Compared to naked eye observations, this method allows for more accurate monitoring of circulation in critically injured or ill patients. 
In a gem of an article, from JoVE Developmental Biology, our authors demonstrate a procedure for microinjecting developing jewel wasps Nasonia vitripennis. The test embryos were removed from their parasitized host pupae, and successfully injected with CRISPR/Cas9 molecules. This procedure overcomes significant challenges in working with jewel wasp embryos and will allow future research groups to perform genome modifications in this and similar organisms. 
Our final article heats things up, with laboratory experiments simulating the formation of a lava-phase following a nuclear reactor core meltdown. In JoVE Chemistry, our authors heat real nuclear fuel, cladding, and containment materials to temperatures beyond 3,000 degrees Kelvin. These experiments answer key questions related to core meltdown, and contribute to the prevention of severe accidents in nuclear power plants. That's hot science!
You've just had a sneak peek of the December 2017 issue of JoVE. Visit our website to see the full-length articles, plus many more, in JoVE: The World's Premier Video Journal.

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JoVE Monthly Highlights December 2017

Here's a look at what's coming up in the December 2017 issue of JoVE: The World's Premier Video Journal.
From a flurry of fantastic articles for December, ...

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Video: JoVE Monthly Highlights December 2017

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

JoVE Monthly Highlights December 2017

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