polymerase-chain-reaction-dot-blot-hybridization-for-leptospira

Polymerase Chain Reaction and Dot-Blot Hybridization for <i>Leptospira</i> Detection in Water Samples

The dot-blot is a simple, fast, sensitive, and versatile technique that enables the identification of minimal quantities of DNA specifically targeted by ...

General Hospital “Dr. Manuel Gea González”

Uropathogenic Escherichia coli (UPEC) strains cause urinary tract infections and employ type 1 and P pili in colonization of the bladder and kidney, respectively. Most intestinal and extra-intestinal E. coli strains produce a pilus called E. coli common pilus (ECP) involved in cell adherence and biofilm formation. However, the contribution of ECP to the interaction of UPEC with uroepithelial cells remains to be elucidated. Here, we report that prototypic UPEC strains CFT073 and F11 mutated in the major pilin structural gene ecpA are significantly deficient in adherence to cultured HeLa (cervix) and HTB-4 (bladder) epithelial cells in vitro as compared to their parental strains. Complementation of the ecpA mutant restored adherence to wild-type levels. UPEC strains produce ECP upon growth in Luria-Bertani broth or DMEM tissue culture medium preferentially at 26°C, during incubation with cultured epithelial cells in vitro at 37°C, and upon colonization of mouse bladder urothelium ex vivo. ECP was demonstrated on and inside exfoliated bladder epithelial cells present in the urine of urinary tract infection patients. The ability of the CFT073 ecpA mutant to invade the mouse tissue was significantly reduced. The presence of ECP correlated with the architecture of the biofilms produced by UPEC strains on inert surfaces. These data suggest that ECP can potentially be produced in the bladder environment and contribute to the adhesive and invasive capabilities of UPEC during its interaction with the host bladder. We propose that along with other known adhesins, ECP plays a synergistic role in the multi-step infection of the urinary tract.

Production of the Escherichia coli common pilus by uropathogenic E. coli is associated with adherence to HeLa and HTB-4 cells and invasion of mouse bladder urothelium.

The presentation of clinical leptospirosis has been historically associated with animal workers, slaughterhouse workers and medical veterinarians. This association has shifted to be related to flooding events and outdoor activities; few cases are related to high-risk factors found in immunosuppressed patients. Scarcely a handful of cases have serological evidence of immune response against Leptospira serovar Bratislava representing serogroup Australis, a serovar associated with poor reproductive performance in swine and horses, and recently with cats.

Leptospirosis in an asplenic patient -case report.

The causative agents of leprosy are the well-known  and the newly discovered . This agent was found in 2008, and it was found to be the cause of diffuse lepromatous leprosy in two Mexican patients.

Identification of  and  in Formalin-Fixed and Paraffin-Embedded Skin Samples from Mexico.

Sporotrichosis is a fungal infection caused by species of the  genus. Presently, the prevalence of sporotrichosis in the Americas is unknown, so this study aims to analyze the cases reported in the past 10 years.

Erika Margarita Carrillo-Casas

Department of Molecular Biology and Histocompatibility

General Hospital “Dr. Manuel Gea González”

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

Erika Margarita Carrillo-Casas

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Molecular Biology and Histocompatibility

General Hospital “Dr. Manuel Gea González”

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

Department of Molecular Biology and Histocompatibility