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Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

It is generally accepted that in vitro cell material interaction is a useful criterion in the evaluation of dental material biocompatibility. The ...

UMR CNRS 5615, Université Lyon1

Hospices Civils de Lyon

The purpose of this study was to investigate the role of light parameters on nanohybrid composite curing. Two nanohybrid resins were cured by two light-emitting diode (LED) devices and by one quartz-tungsten-halogen (QTH) device using different combinations of energy density and power density (8 J cm(-2) and 400 mW cm(-2); 8 J cm(-2) and 1,000 mW cm(-2); 16 J cm(-2) and 400 mW cm(-2); and 16 J cm(-2)-1,000 mW cm(-2)). The effects of these combinations on polymerization were assessed by measuring the Vickers microhardness. Data differed for the two composites and varied according to the light parameters and the nature of the curing device. For both resins, an energy density of 16 J cm(-2) yielded the best microhardness values at both the top and the bottom of the sample, independently of the power density. When using a lower energy density of 8 J cm(-2), a modulated power density was required to achieve proper curing at the bottom of the sample: 8 J cm(-2) and 400 mW cm(-2) induced greater values at the bottom surface. At an energy density of 16 J cm(-2), the power density was not relevant (no significant differences were found between 400 and 1,000 mW cm(-2)), except when the emission spectra of the light-curing units (LCUs) did not match exactly with the absorption spectra of the photoinitators included in the resins (greatest values with 16 J cm(-2) and 1,000 mW cm(-2)). These results suggest that above a certain energy density threshold, the power density may not significantly influence the polymerization kinetics.

Influence of light energy and power density on the microhardness of two nanohybrid composites.

The present work intends to evaluate the use of immediate loaded orthodontic screws in a growing model, and to study the specific bone response. Thirty-two screws (half of stainless steel and half of titanium) were inserted in the alveolar bone of 8 growing pigs. The devices were immediately loaded with a 100 g orthodontic force. Two loading periods were assessed: 4 and 12 weeks. Both systems of screws were clinically assessed. Histological observations and histomorphometric analysis evaluated the percent of "bone-to-implant contact" and static and dynamic bone parameters in the vicinity of the devices (test zone) and in a bone area located 1.5 cm posterior to the devices (control zone). Both systems exhibit similar responses for the survival rate; 87.5% and 81.3% for stainless steel and titanium respectively (p = 0.64; 4-week period), and 62.5% and 50.0% for stainless steel and titanium respectively (p = 0.09; 12-week period). No significant differences between the devices were found regarding the percent of "bone-to-implant contact" (p = 0.1) or the static and dynamic bone parameters. However, the 5% threshold of "bone-to-implant contact" was obtained after 4 weeks with the stainless steel devices, leading to increased survival rate values. Bone in the vicinity of the miniscrew implants showed evidence of a significant increase in bone trabecular thickness when compared to bone in the control zone (p = 0.05). In our study, it is likely that increased trabecular thickness is a way for low density bone to respond to the stress induced by loading.

In vivo evaluation of immediately loaded stainless steel and titanium orthodontic screws in a growing bone.

Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

Kerstin Gritsch

Laboratoire des Multimatériaux et Interfaces

UMR CNRS 5615, Université Lyon1

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Kerstin Gritsch

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Laboratoire des Multimatériaux et Interfaces

UMR CNRS 5615, Université Lyon1

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Laboratoire des Multimatériaux et Interfaces