Inducing Massive Pontine Hemorrhage in a Rat Model by Autologous Blood Injection

Begin with an anesthetized rat secured on a stereotaxic frame holding an anticoagulant-coated Hamilton syringe. 

The rat’s skull is exposed, with a hole over the brain’s pontine region. 

Insert an acupuncture assembly into the tail vein, withdraw blood, and transfer it to a tube. 

Set the syringe to withdrawal mode and aspirate the blood. 

Switch to infusion mode and insert the syringe through the hole, reaching the pontine region.

Inject a small volume of the autologous blood. Hold the syringe to prevent backflow. The injected blood clots over time.

After an interval, retract the syringe to the original position. Inject the remaining blood into the same region, holding for another interval before retracting.

The dual-injection method prevents blood from spreading to the surrounding area.

The infused blood enlarges the clot, exerting localized pressure on the surrounding vessels, causing them to rupture.

This results in significant blood loss and induces a massive pontine hemorrhage.