Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction AssayCrystal M. Gigante 1, Vaughn Wicker 1, Rene Edgar Condori 1,2, Kimberly Wilkins 1, Yu Li 1
1Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, 2Goldbelt Professional Services
This protocol demonstrates the pan-lyssavirus LN34 real-time reverse transcription-polymerase chain reaction (RT-PCR) assay from tissue collection to result interpretation, including updates to primer sequences and formulations to improve assay performance for some non-rabies lyssaviruses and lagomorphs. We also demonstrate the assay setup for a single-well LN34 multiplexed (LN34M) format.
