JoVE Journal

Immunology and Infection

This content is Open Access.

Utilizzando cristallografia a raggi X, Biofisica, e saggi funzionali per determinare i meccanismi di amministrazione delle cellule T del recettore Riconoscimento di cancro antigeni

Here, we describe methods that we commonly employ in the laboratory to determine how the nature of the interaction between the T-cell receptor and tumor antigens, presented by human leukocyte antigens, governs T-cell functionality; these methods include protein production, X-ray crystallography, biophysics, and functional T-cell experiments.

Capitoli in questo video

0:05

Title

1:05

T-cell Receptor (TCR) and Human Leukocyte Antigen-resticted Tumor-derived Peptide (pHLA) Refolding and Purification by Fast Protein Liquid Chromatography (FPLC)

3:43

Surface Plasmon Resonance (SPR) Equilibrium Binding and Thermodynamic Analyses

5:55

Protein Crystallization and X-ray Diffraction

7:39

Results: Molecular Analysis of gp100280-288 Recognition by CD8+ T-cells

8:59

Conclusion

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