Sviluppo e validazione di un metodo quantitativo per PCR Equid herpesvirus-2 Diagnostica in respiratorie Fluidi

Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.