A Protocol for Explant Cultures of IDH1-mutant Diffuse Low-grade GliomasKasandra Aguilar-Cázarez 1, Donovan Pineau 1, Leonor Garcia 1, Guo-Hao Huang 2, Sheng-Qing Lv 2, Hugues Duffau 1,3, Jean-Philippe Hugnot 1,4
1Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 2Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University of Chongqing, 3Department of Neurosurgery, Gui de Chauliac Hospital, Montpellier University Medical Center, 4Jingfeng laboratories of Chongqing
IDH1-mutant diffuse low-grade gliomas are rare tumors that are difficult to culture. We introduce a simple, non-enzymatic explant method that preserves IDH1-mutant protein expression and sustains cultures for months. Importantly, this technique works with cryopreserved tumors, offering a valuable resource for glioma research.
