JoVE Journal

Developmental Biology

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Nanyang Technological University

הערכה תת סוגי Cardiomyocyte בעקבות תמלול פקטור בתיווך תכנות מחדש של פיברובלסטים עכבר עובריים

This manuscript describes a step-by-step protocol for the generation and quantification of diverse reprogrammed cardiac subtypes using a retrovirus-mediated delivery of Gata4, Hand2, Mef2c, and Tbx5.

Chapters in this video

0:05

Title

0:40

Retrovirus Production in Plat-E Cells

2:04

Hcn4-GFP Mouse Embryonic Fibroblast Seeding

2:57

Transduction and Reprogramming of Mouse Embryonic Fibroblasts

4:30

Immunostaining of Reprogrammed MEFs

6:01

Identification of Cardiac Subtypes Using Confocal Microscopy

6:56

Quantification of Sarcomere+ Cells

7:32

Results: Identification of Cardiomyocyte Sub-populations

8:44

Conclusion

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