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Nanyang Technological University

זיהוי של איתות תאיים אירועים מושרה תאים קיימא על ידי אינטראקציה עם תאים שכנים מוות של תאים אפופטוטיים שעברו

Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.

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Chapters in this video

0:05

Title

0:49

Preparation of Apoptotic BU.MPT Cells

3:03

Preparation of Necrotic BU.MPT Cells

4:39

Preparation of BU.MPT Responder Cells

5:27

Stimulation of Responder Cells with Apoptotic and Necrotic Cells

7:03

Results: Inhibition of Phosphorylation of GSK3α/β in BU.MPT Responder Cells Following Exposure to Apoptotic Cells Requires Direct Cell-cell Interaction but Is Independent of Phagocytosis

8:08

Conclusion

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