JoVE Journal

Biochemistry

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Nanyang Technological University

חיישן מבוסס-Polyaniline של חומצות גרעין

Nucleic acids are common analytes for assessing biological systems; however, bias from enzymatic manipulation can cause concern. Here a method is described for label-free detection of nucleic acids using polyaniline. This sensitive, cost-effective sensor technology can distinguish single nucleotide differences between molecules.

Chapters in this video

0:05

Title

0:55

Processable PANI Synthesis

2:40

PANI-probe Mixing and UV Irradiation and Hybridization of PANI-probe

4:21

Emission Steady State Fluorescence Measurement and Fluorescence Microscopy Measurement of Hybridized Duplex

5:45

Results: Use of PANI Fluorescence to Detect Nucleic Acid Hybridization

6:53

Conclusion

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