JoVE Journal

Bioengineering

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Nanyang Technological University

עיבוד אנזימתי תענית חלבונים עבור איתור MS עם microreactor זרימה דרך

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09:49 min

April 6th, 2016

April 6th, 2016


A quick protocol for proteolytic digestion with an in-house built flow-through tryptic microreactor coupled to an electrospray ionization (ESI) mass spectrometer is presented. The fabrication of the microreactor, the experimental setup and the data acquisition process are described.

Chapters in this video

0:05

Title

0:41

Preparation of the Capillary Microreactor

3:07

Experimental Setup

5:03

Sample Loading, Proteolytic Digestion, and Elution for MS Analysis

7:05

Data Processing

7:50

Results: Mass Spectrometry Protein Detection Using a Flow-through Microreactor

8:11

Conclusion

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