JoVE Journal
Chemistry
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Nanyang Technological University13.4K Views
•
09:55 min
•
July 4th, 2016
DOI :
July 4th, 2016
•0:05
Title
0:46
In Vivo Membrane-targeted Expression
3:09
Isolation from Membrane Fractions
4:41
Purification Using Immobilized Metal-affinity Chromatography (IMAC), Ion-exchange, and Gel Filtration
7:09
Heat Modifiability Assay
8:35
Results: Crystals to Structure Determination for the Putative Vaccine Target YiuR from Yersinia pestis
9:29
Conclusion
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