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Nanyang Technological University

התאמת שינויים גנטיים ספציפי DNA מתילציה עם ביטוי ותעתיק פעילות של astrocytic

DNA methylation is capable of maintaining stable levels of gene expression as well as allowing for dynamic changes in gene expression in response to a variety of stimuli. We detail techniques that allow the study of gene-specific changes in DNA methylation and the effect of these changes on gene expression.

Chapters in this video

0:05

Title

1:25

RNA and DNA Isolation from an Enriched Astrocytic Population using FACS

4:03

Assessing DNA Methylation Status of a Gene Using Methylation-sensitive High Resolution Melt Analysis

5:26

Assessing Hyper-methylated Promoter Activity using a Luciferase Assay

8:50

Results: DNA Methylation Analysis of KCNJ10 in Astrocytes

10:42

Conclusion

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