Evaluación conformacional del VIH-1 glicoproteínas de la envoltura trimérica El uso de un ELISA ensayo basado en células

Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env antibodies.