biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

neuronal culture techniques

This collection features a variety of neuronal culture techniques, ranging from basic neuron cultures to advanced protocols for generating neural progenitors. It covers specific neuron cultures that employ three-dimensional scaffolds or microfluidics, and differentiation into brain organoids and networks. Techniques also encompass co-cultures to study neuron-glia interactions and synapse formation, among other approaches for exploring neuronal behavior and neurogenesis in vitro.

Neuronal Culture Techniques

neurophysiology

This collection presents methodologies for studying neural function and behavior. It features direct measurement techniques for recording electrical activities from neurons in diverse settings, and optical techniques for visualizing dynamic neuronal processes, tracking real-time changes, and examining spatial relationships within neural circuits. Additionally, it provides various stimulation methods using electrical, magnetic, and optogenetic tools to explore and modulate neural circuit responsiveness.

Neurophysiology

neuroimaging

This collection offers advanced methodologies for investigating brain structure, functional connectivity, and molecular dynamics in the nervous system. Focusing on in vitro and in vivo models, advanced imaging, and analytical techniques, these protocols equip researchers with innovative tools to study neural activity and pathologies across diverse areas.

Neuroimaging

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neuropathology

This collection offers advanced methodologies for investigating molecular mechanisms, structural changes, and functional disruptions in nervous system disorders. Focusing on in vitro and in vivo models, advanced imaging, and molecular analyses, these protocols equip researchers with innovative tools to study brain and neural pathologies across diverse areas.

Neuropathology

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electrophysiology techniques

Electrophysiology Techniques

imaging and optical techniques

Imaging and Optical Techniques

stimulation and modulation techniques

Stimulation and Modulation Techniques

in vivo recording of intracellular signals from a single neuron

in-vivo-recording-of-intracellular-signals-from-a-single-neuron

In Vivo Recording of Intracellular Signals from a Single Neuron

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

establishing a whole-cell configuration through the patch clamp method

establishing-a-whole-cell-configuration-through-the-patch-clamp-method

Establishing a Whole-Cell Configuration through the Patch Clamp Method

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Slice ...

measuring the electrophysiological activity of neuronal networks using micro-electrode arrays

measuring-electrophysiological-activity-neuronal-networks-using-micro

Measuring the Electrophysiological Activity of Neuronal Networks Using Micro-Electrode Arrays

1. Differentiation of&#160;rtTA/Ngn2-positive hiPSCs to Neurons on 6-well MEAs and Glass Coverslips
NOTE:&#160;In this protocol, the details are provided ...

patch-clamp recordings from the dendrite of a dopaminergic neuron in a brain slice

patch-clamp-recordings-from-dendrite-dopaminergic-neuron-brain

Patch-Clamp Recordings from the Dendrite of a Dopaminergic Neuron in a Brain Slice

analyzing neural activity in primary murine spiral ganglion neurons using multielectrode arrays

analyzing-neural-activity-primary-murine-spiral-ganglion-neurons

Analyzing Neural Activity in Primary Murine Spiral Ganglion Neurons Using Multielectrode Arrays

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Electrophysiological ...

applying noise currents to medial vestibular nucleus neurons using whole-cell patch-clamp technique

applying-noise-currents-to-medial-vestibular-nucleus-neurons-using

Applying Noise Currents to Medial Vestibular Nucleus Neurons Using Whole-Cell Patch-Clamp Technique

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Animals
NOTE: Mice were obtained ...

drug microinjection with electrophysiological recording in a rat brain

drug-microinjection-with-electrophysiological-recording-in-a-rat-brain

Drug Microinjection with Electrophysiological Recording in a Rat Brain

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review ...

recording whole-cell currents with gaba puffing in a mouse brain slice

recording-whole-cell-currents-with-gaba-puffing-in-a-mouse-brain-slice

Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Preparation of Solutions

	...

establishing a whole-cell patch clamp for electrophysiological recording from a flat-mount mouse retina

establishing-whole-cell-patch-clamp-for-electrophysiological

Establishing a Whole-Cell Patch Clamp for Electrophysiological Recording from a Flat-Mount Mouse Retina

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. External and Internal Solutions

	...

electrophysiological recordings for assessing neuronal regenerations in co-cultured spinal cord slices

electrophysiological-recordings-for-assessing-neuronal-regenerations

Electrophysiological Recordings For Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Mounting Spinal Cord Tissue ...

utilizing multielectrode arrays to measure synaptic physiology in 3d coculture spheres

utilizing-multielectrode-arrays-to-measure-synaptic-physiology-3d

Utilizing Multielectrode Arrays to Measure Synaptic Physiology in 3D Coculture Spheres

Measurement of Live Synaptic Physiology with Multielectrode Arrays (MEAs)
1. Prepare MEAs for cell culture.

	Clean the surface of each MEA with 1 mL of ...

studying the excitability of fluorescent neurons using a whole-cell patch clamp

studying-excitability-fluorescent-neurons-using-whole-cell-patch

Studying the Excitability of Fluorescent Neurons using a Whole-Cell Patch Clamp

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Electrophysiology
NOTE: The ...

measuring brain glutamate levels under anesthesia in neonatal piglets using a microelectrode array

measuring-brain-glutamate-levels-under-anesthesia-neonatal-piglets

Measuring Brain Glutamate Levels Under Anesthesia in Neonatal Piglets Using a Microelectrode Array

recording neural activity of unfolded hippocampal tissue using penetrating microelectrode array

recording-neural-activity-unfolded-hippocampal-tissue-using

Recording Neural Activity of Unfolded Hippocampal Tissue Using Penetrating Microelectrode Array

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review.
1. Solutions for Surgery and ...

preparing a rat model to assess visual pathway integrity using an electrophysiology setup

preparing-rat-model-to-assess-visual-pathway-integrity-using-an

Preparing a Rat Model to Assess Visual Pathway Integrity Using an Electrophysiology Setup

implantation of electrodes into a mouse cortex for electrocorticographic recordings

implantation-electrodes-into-mouse-cortex-for-electrocorticographic

Implantation of Electrodes into a Mouse Cortex for Electrocorticographic Recordings

1. ECoG/EMG electrode implantation

	Using straight Kelly forceps, slowly screw one ECoG electrode (with straight gold wire) in the vertical axis in the ...

establishing a whole-cell voltage-clamp configuration for electrophysiological recordings in brain slices

establishing-whole-cell-voltage-clamp-configuration-for

Establishing a Whole-Cell Voltage-Clamp Configuration for Electrophysiological Recordings in Brain Slices

a non-invasive approach to study the electrophysiology of motor neurons in zebrafish embryos

a-non-invasive-approach-to-study-electrophysiology-motor-neurons

A Non-invasive Approach to Study the Electrophysiology of Motor Neurons in Zebrafish Embryos

measuring compound muscle action potentials in mouse forelimb muscles in vivo

measuring-compound-muscle-action-potentials-mouse-forelimb-muscles

Measuring Compound Muscle Action Potentials in Mouse Forelimb Muscles In Vivo

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Animal ...

electrophysiological recording of voltage responses of drosophila retinal photoreceptors to light stimuli

electrophysiological-recording-voltage-responses-drosophila-retinal

Electrophysiological Recording of Voltage Responses of Drosophila Retinal Photoreceptors to Light Stimuli

1. Recording from R1-R6 Photoreceptors 

	Always be grounded when operating the microelectrode amplifier (for example by touching the metal surface of the ...

monitoring electrical signals from the afferent neurons in an immobilized zebrafish larva

monitoring-electrical-signals-from-afferent-neurons-an-immobilized

Monitoring Electrical Signals from the Afferent Neurons in an Immobilized Zebrafish Larva

measurement of evoked potassium ion concentration dynamics in coronal hippocampal slices

measurement-evoked-potassium-ion-concentration-dynamics-coronal

Measurement of Evoked Potassium Ion Concentration Dynamics in Coronal Hippocampal Slices

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Preparation of Acute Hippocampal ...

analyzing the glutamate release and clearance at a single neuron synapse in a mouse brain slice

analyzing-glutamate-release-clearance-at-single-neuron-synapse-mouse

Analyzing the Glutamate Release and Clearance at a Single Neuron Synapse in a Mouse Brain Slice

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
NOTE: Recordings from Q175 wild-type ...

recording gamma band oscillations from pedunculopontine nucleus neurons in a brain slice

recording-gamma-band-oscillations-from-pedunculopontine-nucleus

Recording Gamma Band Oscillations From Pedunculopontine Nucleus Neurons in a Brain Slice

in vivo electrophysiological recordings of brain activities from multiple sites in rats

in-vivo-electrophysiological-recordings-brain-activities-from

In Vivo Electrophysiological Recordings of Brain Activities from Multiple Sites in Rats

perforated patch-clamp recording of olfactory sensory neurons in mice

perforated-patch-clamp-recording-of-olfactory-sensory-neurons-in-mice

Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice

recording neural activity in freely moving mice using fiber photometry

recording-neural-activity-in-freely-moving-mice-using-fiber-photometry

Recording Neural Activity in Freely Moving Mice Using Fiber Photometry

an electrophysiological study of retinogeniculate and corticogeniculate synapses in mouse brain slices

an-electrophysiological-study-retinogeniculate-corticogeniculate

An Electrophysiological Study of Retinogeniculate and Corticogeniculate Synapses in Mouse Brain Slices

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Solutions

	Dissection solution
	
...

implanting a wireless bidirectional microelectrode system in a rat brain

implanting-wireless-bidirectional-microelectrode-system-rat

Implanting a Wireless Bidirectional Microelectrode System in a Rat Brain

in vivo measurement of the compound muscle action potential in a rat

in-vivo-measurement-of-the-compound-muscle-action-potential-in-a-rat

In Vivo Measurement of the Compound Muscle Action Potential in a Rat

photostimulation and whole-cell patch clamp recordings of neurons in mouse hippocampal slices

photostimulation-whole-cell-patch-clamp-recordings-neurons-mouse

Photostimulation and Whole-Cell Patch Clamp Recordings of Neurons in Mouse Hippocampal Slices

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Solutions for acute slice ...

simultaneous optical and electrophysiological monitoring of neuronal cells in brain slices

simultaneous-optical-electrophysiological-monitoring-neuronal-cells

Simultaneous Optical and Electrophysiological Monitoring of Neuronal Cells in Brain Slices

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.&#160;&#160;&#160;&#160;&#160;
...

microelectrode array-based assessment of neuronal networks in mouse spinal cord slices

microelectrode-array-based-assessment-neuronal-networks-mouse-spinal

Microelectrode Array-Based Assessment of Neuronal Networks in Mouse Spinal Cord Slices

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1.&#160;In vitro electrophysiology

	...

recording changes in renal sympathetic nerve activity in response to arterial pressure in rats

recording-changes-renal-sympathetic-nerve-activity-response-to

Recording Changes in Renal Sympathetic Nerve Activity in Response to Arterial Pressure in Rats

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Create ...

electrophysiological recordings of reprogrammed neurons in a mouse brain slice

electrophysiological-recordings-reprogrammed-neurons-mouse-brain

Electrophysiological Recordings of Reprogrammed Neurons in a Mouse Brain Slice

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Whole-cell patch-clamp ...

fabrication of flat-interface nerve electrodes for recording neural activity

fabrication-flat-interface-nerve-electrodes-for-recording-neural

Fabrication of Flat-Interface Nerve Electrodes for Recording Neural Activity

1. Electrode Components Preparation

	Gather four electrode components that require precision cutting (laser cutting was used; please refer to the ...

recording of visual evoked potentials via the skull electrodes in a rat model

recording-visual-evoked-potentials-via-skull-electrodes-rat

Recording of Visual Evoked Potentials via the Skull Electrodes in a Rat Model

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. VEP ...

whole-cell patch clamp recording in a substantia gelatinosa neuron of a spinal cord slice

whole-cell-patch-clamp-recording-substantia-gelatinosa-neuron-spinal

Whole-Cell Patch Clamp Recording in a Substantia Gelatinosa Neuron of a Spinal Cord Slice

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review.
1. Preparation of Solutions and ...

recording brain electrical activity in behaving mice using multi-shank linear silicon probes

recording-brain-electrical-activity-behaving-mice-using-multi-shank

Recording Brain Electrical Activity in Behaving Mice Using Multi-Shank Linear Silicon Probes

subdural implantation of a soft electrocorticography array for cortical electrophysiology recording in minipigs

subdural-implantation-soft-electrocorticography-array-for-cortical

Subdural Implantation of a Soft Electrocorticography Array for Cortical Electrophysiology Recording in Minipigs

recording of local field potential in mouse hippocampal-entorhinal cortex slices

recording-local-field-potential-mouse-hippocampal-entorhinal-cortex

Recording of Local Field Potential in Mouse Hippocampal-Entorhinal Cortex Slices

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Transcardial perfusion

	Suspend ...

recording of local field potential from both hemispheres of the mouse brain

recording-local-field-potential-from-both-hemispheres-mouse

Recording of Local Field Potential from Both Hemispheres of the Mouse Brain

analyzing sleep behavior in mice through electroencephalogram and electromyogram recordings

analyzing-sleep-behavior-mice-through-electroencephalogram

Analyzing Sleep Behavior in Mice through Electroencephalogram and Electromyogram Recordings

extracellular recording of electrical activity in the drosophila central nervous system

extracellular-recording-electrical-activity-drosophila-central

Extracellular Recording of Electrical Activity in the Drosophila Central Nervous System

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Dissect and Prepare the Larval ...

monitoring seizure-induced neural electrical activity in brain slices using microelectrode arrays

monitoring-seizure-induced-neural-electrical-activity-brain-slices

Monitoring Seizure-Induced Neural Electrical Activity in Brain Slices Using Microelectrode Arrays

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Preparation of the MEA ...

microrna-mediated inhibition of excitatory postsynaptic currents in mouse hippocampal slices

microrna-mediated-inhibition-excitatory-postsynaptic-currents-mouse

MicroRNA-Mediated Inhibition of Excitatory Postsynaptic Currents in Mouse Hippocampal Slices

1. Assessing the Role of Presynaptic Proteins in Intact Neuronal Circuits by Targeted Stimulation of Knocked-down Neurons with Optogenetics
NOTE: The ...

Encyclopedia Of Experiments - neuroscience