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Jiangsu Normal University

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Cell-Free Protein Synthesis System for Building Synthetic Cells
Mengjiao Cao *1, Mengmeng Yang *1, Yingqiu Li *1, Ke Yue 1, Lulu Shen 1, Lei Kai 1
1School of Life Sciences, Jiangsu Normal University

This protocol describes the Cell-Free Protein Synthesis (CFPS) system used in constructing synthetic cells. It outlines key stages with representative results in different micro-compartments. The protocol aims to establish best practices for diverse laboratories in the synthetic cell community, advancing progress in synthetic cell development.

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