Science Education - Chemistry
Biochemistry
05:26Dialysis: Diffusion Based Separation
Dialysis is a common technique used in biochemistry for separating molecules based on diffusion. In this procedure, a semipermeable membrane allows the ...
08:07Enzyme Assays and Kinetics
Enzyme kinetics describes the catalytic effects of enzymes, which are biomolecules that facilitate chemical reactions necessary for living organisms. ...
07:27MALDI-TOF Mass Spectrometry
Matrix-assisted laser desorption ionization (MALDI) is a mass spectrometry ion source ideal for the analysis of biomolecules. Instead of ionizing ...
07:09Tandem Mass Spectrometry
In tandem mass spectrometry a biomolecule of interest is isolated from a biological sample, and then fragmented into multiple subunits in order to help ...
07:39Protein Crystallization
Protein crystallization, obtaining a solid lattice of biomolecules, elucidates protein structure and enables the study of protein function. ...
07:51Chromatography-based Biomolecule Purification Methods
In biochemistry, chromatography-based purification methods are employed to isolate compounds from a complex mixture. Two such methods used commonly by ...
07:31Two-Dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis (2DGE) is a technique that can resolve thousands of biomolecules from a mixture. This technique involves two distinct ...
08:28Metabolic Labeling
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs ...
06:57Electrophoretic Mobility Shift Assay (EMSA)
The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a ...
08:49Photometric Protein Determination
Measuring the concentration is a fundamental step of many biochemical assays. Photometric protein determination takes advantage of the fact that the more ...
08:42Density Gradient Ultracentrifugation
Density gradient ultracentrifugation is a common technique used to isolate and purify biomolecules and cell structures. This technique exploits the fact ...
08:08Co-Immunoprecipitation and Pull-Down Assays
Co-immunoprecipitation (CoIP) and pull-down assays are closely related methods to identify stable protein-protein interactions. These methods are related ...
07:05Reconstitution of Membrane Proteins
Reconstitution is the process of returning an isolated biomolecule to its original form or function. This is particularly useful for studying membrane ...
06:39Förster Resonance Energy Transfer (FRET)
Förster resonance energy transfer (FRET) is a phenomenon used to investigate close-range biochemical interactions. In FRET, a donor photoluminescent ...
06:58Surface Plasmon Resonance (SPR)
Surface plasmon resonance (SPR) is the underlying optical phenomenon behind label-free biosensors to evaluate the molecular affinity, kinetics, ...
