You have full access to this content through
Nanyang Technological University-- views • 1:25 min
The brain's oxytocin receptor-rich nuclei are clusters of neurons that express oxytocin receptors, which interact with oxytocin, a neuropeptide.
To isolate oxytocin receptor-rich nuclei, begin with a neonatal rat brain.
Place the brain in a mold with a slicing guide. Slice the entire brain to expose different regions with nuclei.
Transfer these slices to a dish and immobilize them.
Add artificial cerebrospinal fluid, or aCSF, to maintain neuronal viability and incubate under gentle rotation.
Using a published brain atlas as a reference, identify anatomical landmarks to locate oxytocin-receptor-rich nuclei within the slices.
Transfer an identified brain slice of interest to a new plate.
Under a microscope, locate the nuclei within the slice and punch it.
Repeat the process for other identified sections to isolate the oxytocin-rich nuclei.
Transfer the isolated nuclei to a tube containing ice-cold protein extraction buffer supplemented with protease and phosphatase inhibitors to preserve the oxytocin receptors.
相关视频
16 Views
相关视频
12.1K Views
相关视频
2.1K Views
相关视频
2.4K Views
相关视频
2.4K Views
相关视频
2.4K Views
版权所属 © 2025 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。