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Honda Research Institute Japan Co., Ltd.

1 ARTICLES PUBLISHED IN JoVE

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Genetics

Direct Liquid-Culture Screening for Evaluating the Production of Heterologous Proteins Using an Auxotrophic Mutant of Aspergillus Oryzae
Satoru Shinkawa 1, Shigenobu Mitsuzawa 1
1Honda Research Institute Japan Co., Ltd.

A direct liquid-culture (DLC) screening method has been developed which significantly reduces the time required for polyethylene glycol (PEG)-mediated protoplast transformation of the filamentous fungus, Aspergillus oryzae, when employed for evaluation of the secretory production of heterologous proteins. This method dramatically increases the throughput of the evaluation protocol.

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