TU Dortmund University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Narendar Kolimi¹, Sanjeev Ghimire¹, Frank Duffy¹, Thomas Peulen², Exequiel Medina³, Hugo Sanabria¹

¹Department of Physics and Astronomy, Clemson University, ²Department of Chemistry and Chemical Biology, TU Dortmund University, ³Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile

Here, we present the protocol to study the local flexibility and dynamics of biomolecules using time-resolved fluorescence anisotropy at the single-molecule level in confocal microscopy mode.