The First Affiliated Hospital of Guangzhou Medical University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Methods for Detecting Cough and Airway Inflammation in Mice

Wenbin Ding¹, Mengxi Luo¹, Zhengyang Lin¹, Zheng Deng^1,2

¹State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, ²Guangzhou National Laboratory

Here, we describe the measurement of cough using a noninvasive and real-time whole-body plethysmography (WBP) system and the normative procedures for harvesting tissue samples of mice and introduce some methods to assess airway inflammation.

Medicine

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis

Qian Jiang^*1, Dongmei Wen^*1, Fang Peng^*2, Wei Hong³, Xin Fu³, Yuanhui Xiong¹, Chongyuan Ren¹, Xuanyi Li¹, Dansha Zhou^1,4, Jian Wang^1,4, Yuqin Chen¹

¹State Key Laboratory of Respiratory Diseases, National Center for Respiratory Medicine, Guangdong Key Laboratory of Vascular Diseases, National Clinical Research Center for Respiratory Diseases, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, ²Department of Critical Care Medicine, The Third Affiliated Hospital of Guangzhou Medical University, ³GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, ⁴Guangzhou laboratory, Guangzhou International Bio Island

Here, the method of inducing pulmonary arterial hypertension associated with pulmonary fibrosis (PF-PH) rat model by injecting bleomycin into the airway is introduced. We also provide a step-by-step approach to validate this animal model.