Okayama University of Science

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Shun-ichi Ito¹, Yoshinori Tanaka¹

¹Biochemistry Unit, Faculty of Veterinary Medicine, Okayama University of Science

Here, we present the methodology for concisely assessing autophagosome marker LC3-II levels in extracellular vesicles (EVs) by immunoblotting. Analysis for LC3-II levels in EVs, autolysosome formation, and omegasome formation suggests the new role of STX6 in the release of LC3-II-positive EVs when autophagosome-lysosome fusion is inhibited.