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Developmental Biology

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Nanyang Technological University

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

6.6K Views

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09:07 min

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February 24th, 2017

DOI :

10.3791/55120-v

February 24th, 2017

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Clement D. Marshall¹, Elizabeth R. Zielins¹, Elizabeth A. Brett¹, Charles P. Blackshear¹, Michael S. Hu¹, Tripp Leavitt¹, Leandra A. Barnes¹, H. Peter Lorenz¹, Michael T. Longaker¹, Derrick C. Wan¹

¹Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine

Summary

Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

Explore More Videos

Adipose derived Stromal Cells

Calvarial Defect

Craniofacial Regeneration

Osteogenic Activity

Regenerative Biology

Subcutaneous Fat

Chapters in this video

0:05

Title

1:16

Harvesting and Isolation of ASCs

3:19

FACS Sorting for BMPR-1B Positive Cells

5:39

Creation of Calvarial Defects and Application of ACS-containing Scaffold

7:03

Results: Bone Regeneration in Response to BMPR-1B-positive ASC

8:02

Conclusion

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