Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
11.0K Views
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14:46 min
•
May 28th, 2015
DOI :
May 28th, 2015
•Chapters in this video
0:05
Title
1:54
Design of Targeting Vectors
2:41
Design and Vector Construction of sgRNAs for CRISPR/Cas9 System
5:20
Design and Construction of Cas9n Double Nickase sgRNA
8:06
Evaluation of sgRNAs by T7 Endonuclease1
11:03
In Silico Prediction of Potential Off Target Sites
12:32
Results: The T7E1 Assay is Used to Evaluate sgRNAs Prior to Transfecting hiPSCs
13:56
Conclusion
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