JoVE Journal

Bioengineering

This content is Open Access.

الزخرفة مطروح السريع لطبقات الخلايا الحية مع التحقيق ميكروفلويديك

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12:19 min

September 15th, 2016

September 15th, 2016


We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.

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Chapters in this video

0:05

Title

6:13

Creating the Hierarchical Hydrodynamic Flow Confinement (hHFC) and Patterning Cell Monolayers Using hHFC

1:16

Microfluidic Probe (MFP) Head and Platform Cleaning and Preparation

8:10

Downstream Processing for Sampling and DNA Amplification

9:46

Results: Rapid Subtractive Patterning of Cell Monolayers Using a Hierachical HFC

10:54

Conclusion

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