JoVE Journal

Medicine

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Nanyang Technological University

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

EUCAST has developed a direct antimicrobial susceptibility testing (AST) protocol for automated blood cultures. However, its dependence on mass spectrometry-based microbial identification can be obviated by using a direct inoculum preparation protocol in an automated microbial identification system. This approach can provide AST reports within 24 h of sample collection.

Chapters in this video

0:00

Introduction

2:15

Standard Inoculum Protocol (SIP) for Bacterial Identification Using an Automated Microbial Identification and Antimicrobial Susceptibility Testing System(aMIAST)

4:22

Direct Inoculum Protocol (DIP) for Bacterial Identification Using an Automated Microbial Identification and Antimicrobial Susceptibility Testing System(aMIAST)

6:52

Antimicrobial Susceptibility Test (AST) Using the EUCAST Rapid Antimicrobial Sensitivity Testing (RAST)

8:00

Quality Control Testing for Rapid Antimicrobial Sensitivity Testing (RAST)

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