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Nanyang Technological University

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

1.3K Views

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08:49 min

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December 1st, 2023

DOI :

10.3791/65388-v

December 1st, 2023

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Jackson C. Wagner¹, Bin Yang¹^,², Zishan Wu¹, Wei Xiong¹^,³^,⁴

¹Department of Chemistry and Biochemistry, UC San Diego, ²State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, ³Materials Science and Engineering Program, UC San Diego, ⁴Department of Electrical and Computer Engineering, UC San Diego

Summary

A multimodal, rapid hyperspectral imaging framework was developed to obtain broadband vibrational sum-frequency generation (VSFG) images, along with brightfield, second harmonic generation (SHG) imaging modalities. Due to the infrared frequency being resonant with molecular vibrations, microscopic structural and mesoscopic morphology knowledge is revealed of symmetry-allowed samples.

Explore More Videos

Multimodal Imaging

Nonlinear Optical Microscopy

Hyperspectral Imaging

Vibrational Sum frequency Generation

Microscopic Properties

Self assembled Samples

Chemical Composition

Molecular Arrangements

Data Acquisition Technology

Second Harmonic Generation

Biophysical Studies

Optical Imaging Techniques

Spectral Resolving Power

Soft Tissues Analysis

Chapters in this video

0:00

Introduction

2:26

Hyperspectral Line‐Scanning VSFG Microscope Setup, Alignment, and Calibration

6:34

Hyperspectral Line‐Scanning VSFG Microscope Data Collection and Analysis

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