JoVE Journal

Biochemistry

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Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.

Chapters in this video

0:00

Introduction

3:09

Cell Culture and Drug Treatment in the Human Embryonic Kidney 293 (HEK293) Cell Line

4:19

Isolation and Normalization of DNA Containing Crosslinked Proteins

7:02

Western Blotting of Digested DNA Samples Containing DNA‐Protein Crosslinks ( DPCs)

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