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Nanyang Technological University

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera

1.0K Views

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04:51 min

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May 26th, 2023

DOI :

10.3791/65278-v

May 26th, 2023

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Sharada D. Mohite¹^,², Yogita P. Patil¹^,², Vikram J. Nichit¹^,², Rakesh S. Joshi¹^,²

¹Biochemical Sciences Division, CSIR-National Chemical Laboratory, ²Academy of Scientific and Innovative Research (AcSIR)

Summary

This protocol describes the obligate feeding assay to evaluate the potentially toxic effect of a phytochemical on the lepidopteran insect larvae. This is a highly scalable insect bioassay, easy to optimize the sublethal and lethal dose, deterrent activity, and physiological effect. This could be used for screening eco-friendly insecticides.

Explore More Videos

Insecticidal Effect

Helicoverpa Armigera

Antifeedant Properties

Pest Management

Synthetic Molecules

Artificial Diet

Feeding Behavior

Developmental Stages

Experimental Conditions

Agricultural Productivity

Phytochemical Evaluation

Chapters in this video

0:00

Introduction

1:36

Preparing a Quercetin‐Containing Chickpea‐Based Artificial Diet for Evaluating its Effect on Helicoverpa armigera Growth

2:26

Feeding Assay to Evaluate the Effects of Quercetin on Helicoverpa armigera Growth and Development

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