JoVE Journal

Biology

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Nanyang Technological University

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

This paper presents the methodology for obtaining and assessing vascular calcification by isolating murine aortas followed by extracting calcified extracellular vesicles to observe the mineralization potential.

Chapters in this video

0:05

Introduction

0:54

Organ Removal

1:50

Bone Removal

2:50

Microdissection

4:48

Isolating and Extracting Extracellular Vesicles from Aortas

5:26

Assessing the Calcification Potential of Extracellular Vesicles with Light Scattering Absorbance

6:11

Assessing the Calcification Mineral Formation of Extracellular Vesicles with Collagen Hydrogels

7:39

Results: Analysis of Vascular Calcification in Collagen Hydrogels

8:23

Conclusion

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