JoVE Journal
Biochemistry
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Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
1.2K Views
•
09:07 min
•
December 23rd, 2022
DOI :
December 23rd, 2022
•Chapters in this video
0:04
Introduction
1:03
Preparation of ConA‐Sepharose Beads and ConA‐Sepharose: Amylopectin Beads
2:19
Starch Excess4 (SEX4) Protein Binding with ConA‐Sepharose: Amylopectin Beads
3:41
Running SDS‐PAGE Gels
4:27
Western Blotting for Chemiluminescence Detection and Data Analysis
6:28
Results: Binding Affinity of SEX4 to ConA‐Sepharose‐Amylopectin Beads
8:18
Conclusion
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ISSN 2578-2037
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