High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
1.8K Views
•
14:03 min
•
March 24th, 2023
DOI :
March 24th, 2023
•Chapters in this video
0:04
Introduction
0:57
Commercially Available Cryopreserved hiPSC‐CMs Plating for Maturation on a Maturation‐Inducing Extracellular Matrix (MECM)
3:02
Human‐Induced Pluripotent Stem Cell‐Derived Cardiomyocytes (hiPSC‐CM) Purification
6:01
Optical Mapping Using Voltage‐Sensitive Dyes (VSDs) and Calcium‐Sensitive Fluorophores (CSFs)
7:32
Optical Mapping Using Genetically Encoded Calcium Indicator (GECI)
8:18
Acquisition of Optical Mapping Data and Analysis
10:45
Results: Robust and Rapid Maturation of Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes (hiPSC‐CMs)
13:12
Conclusion
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