Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
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10:26 min
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October 21st, 2022
DOI :
October 21st, 2022
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Chapters in this video
0:04
Introduction
1:13
Preparation of Cell Suspension
3:24
Live-Dead Analysis of Tumor Spheroids
4:05
Disintegration and Cell Suspension of Tumor Spheroids
5:02
Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads
6:19
Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs
7:28
Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts
9:30
Conclusion
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